If your answer to any of the above questions is yes, you need the new GAT-DStar VERSAPrep 100™. This system will meet your needs for preparing milligrams to grams samples and provide advanced purification capabilities and savings of expensive mobile phases at the same time. The instrument incorporates powerful recycling and solvent saving features not available as standard on other popular Prep systems.

The GAT-DStar VERSAPrep 100™ can save you time and money - two immediate benefits that directly affect your operation’s effectiveness.

Important features of the GAT-DStar VERSAPrep 100™

• The software Solvent Saver™ function is a built-in recycling feature that saves solvent consumption in isocratic methods by automatically re-circulating the mobile phase until a change in absorbance greater than the allowed baseline threshold is detected.

• Closed-loop Recycling of the sample peak using a recycling valve from the column exit (detector) to the head of the column increases the theoretical column length, thus column efficiency. The multiple pass technique results in a superior purification procedure.

• Closed-loop recycling and solvent saving functions reduce solvent consumption by 25 to 70% providing substantial savings to your Prep operations. Additional savings can be achieved using stacked injections and an injection pump.

• Integrated peak detection and fraction collection.
• Small footprint minimizes required bench space.
• System has an optimized low dead volume flow path.
• Optional injection pump allows the Stacked Injection feature of the software to provide for higher sample throughput.
• Completely automated operation.
• Instrument price includes computer loaded with VERSAPrep™ software, and a printer.

unique Stacked Injection feature, the injection pump enables higher sample throughput.

GAT-DStar VERSAPrep 100™ Software: An exciting, user-friendly Windows™ based software provides complete control and programming of the pump; control and programming of the detector, including setting of method wavelength; fraction collection; data acquisition of two detector data channels; monitoring system operating pressure; and activating an independent autosampler and fraction collector. The software program incorporates as standard: solvent saving, closed-loop recycling, stacked injection, and four solvent gradient features that are unique to the GAT-DStar VERSAPrep 100™ system.

Applications
Some typical applications on the GAT-DStar VERSAPrep 100™ system:

Figure 1 - Gradient with Fraction Collection

Figure 1 illustrates a Separation using Gradient Elution combined with Automated Fraction Collection. Figures 2 and 3 are the Gradient Program and the Fraction Collection parameters respectively.

Note in the fraction collection table (Figure 3) the overlap of the window times, which allows for robust operation even when the peak retention time varies slightly.

The result is the same fraction is saved in the same collection vessel every time. Even with variations in peak shape the same peak fractions are collected in the same collection vessel.

Figure 2 - Gradient Program Parameters

Figure 6 - Repetitive Injections and Fraction

GAT-DStar VERSAPrep 100™ with Solvent Saver™ and Recycling

The capability of solvent saving and purification should be of interest to every user of preparative HPLC. The Solvent Saver™ software function, when combined with the recycle valve, allows for intuitive and interactive development of preparative HPLC methods for the purification of intermediates and product, or the isolation of impurities for identification.

Many of the manufacturers of analytical HPLC systems are touting the importance of reducing solvent usage in the analytical lab. Typical analytical flow rates are 1 to 3 ml / min range. Since preparative HPLC flow rates can typically range from 20 to 200 ml / min it seems obvious that a similar ability for prep work would be of great importance.

Solvent Saving:
The GAT-DStar VERSAPrep™ software accomplishes reduction in solvent use in two ways. First, similar to the analytical solvent recyclers, it can automatically re-circulate the mobile phase until a peak elutes and stop the recycling at that time. The GAT-DStar VERSAPrep 100™ goes one step better than the traditional technique. Unlike analytical recyclers, the GAT-DStar VERSAPrep 100™ does not contaminate the mobile phase container with the re-circulated mobile phase. Instead the mobile phase flow is diverted back through the pump and then back on to the column.

Figure 7 - Configuration for Solvent Saving